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Article Dans Une Revue Aggregate Année : 2022

A versatile AIE fluorogen with selective reactivity to primary amines for monitoring amination, protein labeling, and mitochondrial staining

Lianrui Hu
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Tsz Shing Cheung
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Min‐Hui Li
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Résumé

Specific bioconjugation for native primary amines is highly valuable for both chemistry and biomedical research. Despite all the efforts, scientists lack a proper strategy to achieve high selectivity for primary amines, not to mention the requirement of fast response in real applications. Herein, we report a chromone-based aggregation-induced emission (AIE) fluorogen called CMVMN as a self-reporting bioconjugation reagent for selective primary amine identification, and its applications for monitoring bioprocesses of amination and protein labeling. CMVMN is AIE-active and capable of solid-state sensing. Thus, its electrospun films are manufactured for visualization of amine diffusion and leakage process. CMVMN also shows good biocompatibility and potential mitochondria-staining ability, which provides new insight for organelle-staining probe design. Combined with its facile synthesis and good reversibility, CMVMN would not only show wide potential applications in biology, but also offer new possibilities for molecular engineering.
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Dates et versions

hal-03807047 , version 1 (08-10-2022)

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Xinyuan He, Huilin Xie, Lianrui Hu, Pengchao Liu, Changhuo Xu, et al.. A versatile AIE fluorogen with selective reactivity to primary amines for monitoring amination, protein labeling, and mitochondrial staining. Aggregate, 2022, ⟨10.1002/agt2.239⟩. ⟨hal-03807047⟩
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