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Article Dans Une Revue STAR Protocols Année : 2021

Purification of astrocyte subtype based on a double reporter mice approach for downstream transcription profiling

Résumé

Characterizing the molecular signature of a cell subtype leads to a better understanding of cell diversity, as this molecular data can identify new cellular markers and offer insights about cell function. Here, we describe an efficient protocol to separate a subtype of astrocytes, the Olig2-AS, from other glial cells by using a double reporter mouse approach and to determine the transcriptome profile of the Olig2-AS from the postnatal spinal cord using RNA-sequencing analysis.
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Dates et versions

hal-03805200 , version 1 (17-10-2022)

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Marion Aguirrebengoa, David Ohayon. Purification of astrocyte subtype based on a double reporter mice approach for downstream transcription profiling. STAR Protocols, 2021, 2 (4), pp.101009. ⟨10.1016/j.xpro.2021.101009⟩. ⟨hal-03805200⟩
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