Purification of astrocyte subtype based on a double reporter mice approach for downstream transcription profiling
Résumé
Characterizing the molecular signature of a cell subtype leads to a better understanding of cell diversity, as this molecular data can identify new cellular markers and offer insights about cell function. Here, we describe an efficient protocol to separate a subtype of astrocytes, the Olig2-AS, from other glial cells by using a double reporter mouse approach and to determine the transcriptome profile of the Olig2-AS from the postnatal spinal cord using RNA-sequencing analysis.
Origine : Fichiers éditeurs autorisés sur une archive ouverte