Article Dans Une Revue ES Journal of Dental Sciences Année : 2023

Effects of ethylenediaminetetraacetic acid on stem cells from the apical papilla: In vitro study

Résumé

Ethylenediaminetetraacetic acid (EDTA) is used as an irrigant in regenerative endodontic treatment. The present study aimed to investigate the effects of EDTA on stem cells from apical papilla (SCAPs) in vitro.

Overall, SCAPs exhibited mesenchymal stem cell characteristics. EDTA treatment at 2.50% and 1.25% did not significantly exhibit cytotoxicity and alter cell morphology. However, EDTA attenuated cell proliferation and reduced MKI67 mRNA expression in SCAPs. Further, EDTA significantly induced early cell apoptosis at 48 h. Cell migration was delayed with EDTA treatment. After maintaining SCAPs in an osteogenic induction medium, EDTA diminished mineral deposition by SCAPs on day 14.

Fichier principal
Vignette du fichier
S199179022200174X.pdf (1.93 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)
Licence

Dates et versions

hal-03790449 , version 1 (08-01-2025)
hal-03790449 , version 2 (22-10-2025)

Licence

Identifiants

Citer

Suphalak Phothichailert, Benya Sangwisutsai, Dhitinant Rattanakosol, Nutcha Teerapongpaibul, Sirawut Hiran-Us, et al.. Effects of ethylenediaminetetraacetic acid on stem cells from the apical papilla: In vitro study. ES Journal of Dental Sciences , 2023, 18 (1), pp.50-56. ⟨10.1016/j.jds.2022.07.014⟩. ⟨hal-03790449v1⟩
163 Consultations
124 Téléchargements

Altmetric

Partager

  • More