Structural and biochemical evidence for the emergence of a calcium-regulated actin cytoskeleton prior to eukaryogenesis - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Communications Biology Année : 2022

Structural and biochemical evidence for the emergence of a calcium-regulated actin cytoskeleton prior to eukaryogenesis

Laurent Blanchoin

Résumé

Abstract Charting the emergence of eukaryotic traits is important for understanding the characteristics of organisms that contributed to eukaryogenesis. Asgard archaea and eukaryotes are the only organisms known to possess regulated actin cytoskeletons. Here, we determined that gelsolins (2DGels) from Lokiarchaeota (Loki) and Heimdallarchaeota (Heim) are capable of regulating eukaryotic actin dynamics in vitro and when expressed in eukaryotic cells. The actin filament severing and capping, and actin monomer sequestering, functionalities of 2DGels are strictly calcium controlled. We determined the X-ray structures of Heim and Loki 2DGels bound actin monomers. Each structure possesses common and distinct calcium-binding sites. Loki2DGel has an unusual WH2-like motif (LVDV) between its two gelsolin domains, in which the aspartic acid coordinates a calcium ion at the interface with actin. We conclude that the calcium-regulated actin cytoskeleton predates eukaryogenesis and emerged in the predecessors of the last common ancestor of Loki, Heim and Thorarchaeota.
Fichier principal
Vignette du fichier
Akil et al - Communications Biology 2022.pdf (7.61 Mo) Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03776003 , version 1 (13-09-2022)

Identifiants

Citer

Caner Akıl, Linh Tran, Magali Orhant-Prioux, Yohendran Baskaran, Yosuke Senju, et al.. Structural and biochemical evidence for the emergence of a calcium-regulated actin cytoskeleton prior to eukaryogenesis. Communications Biology, 2022, 5 (1), pp.890. ⟨10.1038/s42003-022-03783-1⟩. ⟨hal-03776003⟩
35 Consultations
19 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More