Nucleosome assembly and disassembly pathways in vitro - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue PLoS ONE Année : 2022

Nucleosome assembly and disassembly pathways in vitro

Résumé

Structural fluctuations of nucleosomes modulate the access to internal DNA in eukaryotic cells; clearly characterisation of this fundamental process is crucial to understanding gene regulation. Here we apply PhAST (Photochemical Analysis of Structural Transitions) to monitor at a base pair level, structural alterations induced all along the DNA upon histone binding or release. By offering the first reliable, detailed comparison of nucleosome assembly and disassembly in vitro, we reveal similarities and differences between the two processes. We identify multiple, sequential intermediate states characterised by specific PhAST signals whose localisation and amplitude reflect asymmetries of DNA/histone interactions with respect to the nucleosome pseudo dyad. These asymmetries involve not only the DNA extremities but also regions close to the pseudo dyad. Localisations of asymmetries develop in a consistent manner during both assembly and disassembly processes; they primarily reflect the DNA sequence effect on the efficiency of DNA-histone binding. More unexpectedly, the amplitude component of PhAST signals not only evolves as a function of intermediate states but does so differently between assembly and disassembly pathways. Our observation of differences between assembly and disassembly opens up new avenues to define the role of the DNA sequence in processes underlying the regulation of gene expression. Overall, we provide new insights into how the intrinsic properties of DNA are integrated into a holistic mechanism that controls chromatin structure.
Fichier principal
Vignette du fichier
Shymko2022.pdf (1.58 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03775395 , version 1 (12-09-2022)

Licence

Identifiants

Citer

Akiko Hatakeyama, Yuliia Shymko, Brigitte Hartmann, Romain Retureau, Claude Nogues, et al.. Nucleosome assembly and disassembly pathways in vitro. PLoS ONE, 2022, 17, ⟨10.1371/journal.pone.0267382⟩. ⟨hal-03775395⟩
23 Consultations
37 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More