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Article Dans Une Revue Langmuir Année : 2018

Bioconversion of Lignocellulosic Biomass to Fermentable Sugars by Immobilized Magnetic Cellulolytic Enzyme Cocktails

Karthik Periyasamy
Laishram Santhalembi
  • Fonction : Auteur
Marc Aurousseau
Agnès Boyer
  • Fonction : Auteur
Sivanesan Subramanian

Résumé

Enzyme cocktails of reusable, highly stable cellulolytic enzymes play an inevitable role in bioconversion of biomass to biofuels economically. Cellulase, xylanase and β-1,3-glucanase bound silica-amine functionalized iron oxide magnetic nanoparticles (ISN-CLEAs) were prepared and used as the biocatalyst for the depolymerization of cellulosic biomass into monomeric sugar in the present study. The Fe3O4–NPs and Fe3O4@SiO2–NH2–NPs and ISN-CLEAs had an average hydrodynamic size of 82.2, 86.4, and 976.9 nm, respectively, which was confirmed by dynamic light scattering (DLS). About 97% of protein binding was achieved with 135 mM glutaraldehyde at 10 h of cross-linking time and successful binding was confirmed by Fourier transform infrared spectroscopy (FTIR). The ISN-CLEAs exhibited the highest thermal stability of 95% at 50 °C for 2 h and retained extended storage stability of 97% compared to 60% of its free counterpart. Besides, cross-linking allowed ISN-CLEAs reuse for at least eight consecutive cycles retaining over 70% of its initial activity. ISN-CLEAs exhibited approximately 15% increase in carbohydrate digestibility on sugar cane bagasse and eucalyptus pulp than the free enzyme.

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hal-03773101 , version 1 (08-09-2022)

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Karthik Periyasamy, Laishram Santhalembi, Gérard Mortha, Marc Aurousseau, Agnès Boyer, et al.. Bioconversion of Lignocellulosic Biomass to Fermentable Sugars by Immobilized Magnetic Cellulolytic Enzyme Cocktails. Langmuir, 2018, 34 (22), pp.6546-6555. ⟨10.1021/acs.langmuir.8b00976⟩. ⟨hal-03773101⟩

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