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Article Dans Une Revue Panminerva Medica Année : 2022

Optimizing embryological aspects of oocyte retrieval, oocyte denudation, and embryo loading for transfer

Israel Maldonado Rosas
  • Fonction : Auteur
Christina Anagnostopoulou
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Neha Singh
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Nivita Gugnani
  • Fonction : Auteur
Keerti Singh
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Dimple Desai
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Mahsa Darbandi
Madhumita Manoharan
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Sara Darbandi
Annapoorani Chockalingam
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Sofia I. Leonardi Diaz
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Sajal P. Gupta
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Shinnosuke Kuroda
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Renata Finelli
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Hassan Nooman Sallam
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Kelly Athayde Wirka
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Ashok Agarwal
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Résumé

Oocyte retrieval, oocyte denudation, and embryo transfer are crucial processes during assisted reproduction technology (ART). Air quality in the ART laboratory, temperature, pH of the media used and the time interval between oocyte retrieval and insemination are all critical factors. Anesthesia is required for oocyte retrieval, however, evidence regarding the potential impact of different methods (general anesthesia, conscious sedation, and local anesthesia) on the clinical outcomes is unclear. The optimal timing of oocyte denudation following retrieval has not been established. Regarding the mechanical denudation process, there is a lack of evidence to demonstrate the safest minimum inner diameter of denuding pipettes used to complete the removal of granulosa cells surrounding the oocytes. During embryo transfer, many clinics worldwide flush the catheter before embryo loading, in an attempt to potentially rinse off any toxic agents; however, there is insufficient evidence to show that flushing the embryo transfer catheter before loading increases the success of ART outcome. Considering the serious gaps in knowledge in ART practice, the aim of this review is to provide an updated overview of the current knowledge regarding the various steps and techniques involved in oocyte retrieval, oocyte denudation, and embryo loading for transfer.
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Dates et versions

hal-03772675 , version 1 (08-09-2022)

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Citer

Israel Maldonado Rosas, Christina Anagnostopoulou, Neha Singh, Nivita Gugnani, Keerti Singh, et al.. Optimizing embryological aspects of oocyte retrieval, oocyte denudation, and embryo loading for transfer. Panminerva Medica, 2022, 64 (2), p. 156-170. ⟨10.23736/S0031-0808.22.04675-4⟩. ⟨hal-03772675⟩
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