Synergy between nanozymes and natural enzymes on the hybrid MoS2 nanosheets/graphite microfiber for enhanced voltammetric determination of hydrogen peroxide
Résumé
A biosensor for hydrogen peroxide (H2O2) has been developed based on the use of MoS2 nanosheets and graphite that are assembled to form a microfiber hybrid structure. The MoS2 nanosheets are synthesized in situ on a graphite microfiber. The chemical composition and surface morphology of the microfiber hybrid structure has been characterized. The microfiber is shown to display peroxidase-mimicking activity. In the next step, horseradish peroxidase, methylene blue, and chitosan are co-immobilized on the microfiber electrode. The use of MoS2 nanosheets warrants high electrochemical activity of immobilized enzyme on the electrode surface. The modified microfiber electrode, best operated at a voltage of − 0.3 V (vs. Ag/AgCl), can be used to sense H2O2 with a linear response in the 0.1 to 90 μM concentration range and with a determination limit of 30 nM (at S/N = 3). The good response is attributed to the synergistic enhancement of the synthetic nanozymes (few-layered MoS2 nanosheets) and immobilized natural horseradish peroxidase (HRP). [Figure not available: see fulltext.] © 2020, Springer-Verlag GmbH Austria, part of Springer Nature.
Mots clés
chitosan
disulfide
dyes
reagents
indicators
markers and buffers
graphite
horseradish peroxidase
hydrogen peroxide
immobilized enzyme
methylene blue
molybdenum
molybdenum disulfide
nanomaterial
catalysis
chemistry
electrochemical analysis
enzymology
genetic procedures
horseradish
oxidation reduction reaction
procedures
reproducibility
Armoracia
Biosensing Techniques
Catalysis
Chitosan
Disulfides
Electrochemical Techniques
Enzymes
Immobilized
Graphite
Horseradish Peroxidase
Hydrogen Peroxide
Indicators and Reagents
Methylene Blue
Molybdenum
Nanostructures
Oxidation-Reduction
Reproducibility of Results
Loading...