Article Dans Une Revue Chemistry - A European Journal Année : 2022

Cellular Detection of a Mitochondria Targeted Brominated Vinyl Triphenylamine Optical Probe (TP−Br) by X‐Ray Fluorescence Microscopy

Résumé

Triphenylamine (TP) derivatives such as two-branch cationic vinylbenzimidazolium triphenylamine TP−2Bzim are promising turn-on fluorescent probes suitable for two-photon imaging, labelling mitochondria in live cells. Here, we designed two TP−2Bzim derivatives as bimodal probes suitable for X-ray fluorescence imaging. The conjugation of the TP core with a rhenium tricarbonyl moiety in the TP−RePyta probe altered the localisation in live cells from mitochondria to lysosomes. The introduction of bromine on the TP core generated the TP−Br probe retaining good photophysical properties and mitochondria labelling in live cells. The influence of calcium channels in the uptake of TP−Br was studied. Synchrotron Radiation X-ray Fluorescence (SXRF) imaging of bromine enabled the detection of TP−Br and suggested a negligible presence of the probe in an unbound state in the incubated cells, a crucial point in the development of these probes. This study paves the way towards the development of TP probes as specific organelle stainers suitable for SXRF imaging

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hal-03768555 , version 1 (04-09-2022)

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Sounderya Nagarajan, Florent Poyer, Laura Fourmois, Delphine Naud-Martin, Kadda Medjoubi, et al.. Cellular Detection of a Mitochondria Targeted Brominated Vinyl Triphenylamine Optical Probe (TP−Br) by X‐Ray Fluorescence Microscopy. Chemistry - A European Journal, 2022, 28 (15), pp.e202104424. ⟨10.1002/chem.202104424⟩. ⟨hal-03768555⟩
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