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Chapitre D'ouvrage Année : 2022

Studying Cell Division Plane Positioning in Early-Stage Embryos

Résumé

Unraveling the mechanisms that govern division plane orientation is a major challenge to understand plant development. In this respect, the Arabidopsis early embryo is a model system of choice since embryogenesis is relatively simple and cell division planes orientation is highly predictable. Here we present an integrated set of protocols to study 3D cell division patterns in early-stage Arabidopsis embryos that combine both cellular and sub-cellular localization of selected protein markers with spatial organization of cells, cytoskeleton, and nuclei.
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Dates et versions

hal-03766433 , version 1 (01-09-2022)

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Katia Belcram, Jean-Christophe Palauqui, Martine Pastuglia. Studying Cell Division Plane Positioning in Early-Stage Embryos. Plant Cell Division, 2382, Springer US, pp.141-154, 2022, Methods in Molecular Biology, 978-1-0716-1743-4. ⟨10.1007/978-1-0716-1744-1_9⟩. ⟨hal-03766433⟩
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