BODIPY-Ethynylestradiol molecular rotors as fluorescent viscosity probes in endoplasmic reticulum - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-...] Année : 2022

BODIPY-Ethynylestradiol molecular rotors as fluorescent viscosity probes in endoplasmic reticulum

Résumé

Due to their capability for sensing changes in viscosity, fluorescent molecular rotors (FMRs) have emerged as potential tools to develop several promising viscosity probes; most of them, however, localize non-selectively within cells, precluding changes in the viscosity of specific cellular microdomains to be studied by these means. Following previous reports on enhanced fluorophore uptake efficiency and selectivity by incorporation of biological submolecular fragments, here we report two potential BODIPY FMRs based on an ethynylestradiol spindle, a non-cytotoxic semisynthetic estrogen well recognized by human cells. A critical evaluation of the potential of these fluorophores for being employed as FMRs is presented, including the photophysical characterization of the probes, SXRD studies and TD-DFT computations, as well as confocal microscopy imaging in MCF-7 (breast cancer) cells.

Dates et versions

hal-03763207 , version 1 (29-08-2022)

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Diego Martínez-Bourget, Erika Rocha, Pablo Labra-Vázquez, Rosa Santillan, Benjamín Ortiz-López, et al.. BODIPY-Ethynylestradiol molecular rotors as fluorescent viscosity probes in endoplasmic reticulum. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy [1994-..], 2022, 283, pp.121704. ⟨10.1016/j.saa.2022.121704⟩. ⟨hal-03763207⟩
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