A Flexible and Original Architecture of Two Unrelated Zinc Fingers Underlies the Role of the Multitask P1 in RYMV Spread - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Molecular Biology Année : 2022

A Flexible and Original Architecture of Two Unrelated Zinc Fingers Underlies the Role of the Multitask P1 in RYMV Spread

Résumé

Viruses of the sobemovirus genus are plant viruses, most of which generate very important agricultural and financial losses. Among them, the rice yellow mottle virus (RYMV) is one of the most damaging pathogens devastating rice fields in Africa. RYMV infectivity and propagation rely on its protein P1, identified as a key movement and potential long-distance RNA silencing suppressor. Here we describe P1′s complete 3D structure and dynamics obtained by an integrative approach combining X-Ray crystallography and NMR spectroscopy. We show that P1 is organized in two semi-independent and topologically unrelated domains, each harboring an original zinc finger. The two domains exhibit different affinities for zinc and sensitivities to oxidoreduction conditions, making the C-terminal P1 region a potential labile sensor of the plant redox status. An additional level of regulation resides on the capacity of P1 to oligomerize through its N-terminal domain. Coupling P1 structure information with site-directed mutagenesis and plant functional assays, we identified key residues in each zinc domain essential for infectivity and spread in rice tissues. Altogether, our results provide the first complete structure of a sobemoviral P1 movement protein and highlight structural and dynamical properties that may serve RYMV functions to infect and invade its host plant.
Fichier principal
Vignette du fichier
Poignavent et al 2022 P1rymv structure-function in JMB.pdf (2.8 Mo) Télécharger le fichier
Origine : Accord explicite pour ce dépôt

Dates et versions

hal-03758984 , version 1 (23-11-2022)

Licence

Paternité

Identifiants

Citer

Vianney Poignavent, François Hoh, Guillaume Terral, Yinshan Yang, François-Xavier Gillet, et al.. A Flexible and Original Architecture of Two Unrelated Zinc Fingers Underlies the Role of the Multitask P1 in RYMV Spread. Journal of Molecular Biology, 2022, 434 (16), pp.167715. ⟨10.1016/j.jmb.2022.167715⟩. ⟨hal-03758984⟩
108 Consultations
63 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More