Article Dans Une Revue Biochemical and Biophysical Research Communications Année : 2020

Interstitial flow regulates in vitro three-dimensional self-organized brain micro-vessels

Résumé

Cell culture under medium flow has been shown to favor human brain microvascular endothelial cells function and maturation. Here a three-dimensional in vitro model of the human brain microvasculature, comprising brain microvascular endothelial cells but also astrocytes, pericytes and a collagen type I microfiber e fibrin based matrix, was cultured under continuous medium flow in a pressure driven microphysiological system (10 kPa, in 60e30 s cycles). The cells self-organized in micro-vessels perpendicular to the shear flow. Comparison with static culture showed that the resulting interstitial flow enhanced a more defined micro-vasculature network, with slightly more numerous lumens, and a higher expression of transporters, carriers and tight junction genes and proteins, essential to the bloodbrain barrier functions.

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hal-03740372 , version 1 (26-08-2022)

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Agathe Figarol, Marie Piantino, Tomomi Furihata, Taku Satoh, Shinji Sugiura, et al.. Interstitial flow regulates in vitro three-dimensional self-organized brain micro-vessels. Biochemical and Biophysical Research Communications, 2020, 533 (3), pp.600 - 606. ⟨10.1016/j.bbrc.2020.09.061⟩. ⟨hal-03740372⟩
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