Anisotropic nanoparticles (NP), including nanodiscs, nanorods, nanotubes, etc., are used extensively in fields like medicine (fluorescent enhancers, tumor markers and light receptors), electronics (molecular electronic devices, sources of local heating) and a number of others. Self-assemblies of these NPs are also applied in the manufacture of advanced materials in which the size and shape of anisotropic NPs have a significant impact on their properties and, as a consequence, have an influence on their efficiency in the final application. Consequently, being able to fully comprehend NP geometrical dimensions (aspect ratio, length, diameter) at various stages of their synthesis and application is crucial.