Molecular and functional characterization of the mouse intrinsic cardiac nervous system - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Heart Rhythm Année : 2022

Molecular and functional characterization of the mouse intrinsic cardiac nervous system

Résumé

Background : The intrinsic cardiac nervous system (ICNS) refers to clusters of neurons, located within the heart, that participate in the neuronal regulation of cardiac functions and that are involved in the initiation of cardiac arrhythmias. Therefore, deciphering its role in cardiac physiology and physiopathology is mandatory. Objective : The aim of this study was to provide a phenotypic, electrophysiological, and pharmacological characterization of the mouse ICNS, which is still poorly characterized. Methods Global cardiac innervation and phenotypic diversity were investigated using immunohistochemistry on cleared murine hearts and on tissue sections. The patch clamp technique was used for the electrophysiological and pharmacological characterization of isolated mouse intracardiac neurons. Results : We have identified the expression of 7 distinct neuronal markers within the mouse ICNS, thus proving the neurochemical diversity of this network. Of note, it was the first time that the existence of neurons expressing the calcium-binding protein calbindin, neuropeptide Y, and cocaine and amphetamine regulated transcript peptide was described in the mouse. Electrophysiology studies also revealed the existence of 4 different neuronal populations on the basis of their electrical behavior. Finally, we showed that these neurons can be modulated by several neuromodulators. Conclusion : This study showed that the mouse ICNS presents a molecular and functional complexity similar to other species and is therefore a suitable model to decipher the role of individual neuronal subtypes regarding the modulation of cardiac function and the initiation of cardiac arrhythmias.
Fichier principal
Vignette du fichier
Molecular and functional characterization of the.pdf (3.85 Mo) Télécharger le fichier
Origine : Publication financée par une institution
Licence : CC BY NC ND - Paternité - Pas d'utilisation commerciale - Pas de modification

Dates et versions

hal-03715785 , version 1 (30-03-2023)

Licence

Paternité - Pas d'utilisation commerciale - Pas de modification

Identifiants

Citer

Guénaëlle Lizot, Côme Pasqualin, Audrey Tissot, Stephane Pagès, Jean-François Faivre, et al.. Molecular and functional characterization of the mouse intrinsic cardiac nervous system. Heart Rhythm, 2022, ⟨10.1016/j.hrthm.2022.04.012⟩. ⟨hal-03715785⟩
36 Consultations
18 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More