Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C) - Archive ouverte HAL Access content directly
Journal Articles Methods in Molecular Biology Year : 2021

Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C)

Abstract

Chromosome conformation capture and its variants interrogate population-average chromatin structure at a higher resolution and throughput than microscopic methods. Capture Hi-C is a variant tailored for the simultaneous assessment of all interactions with thousands of specific bait sequences, so is particularly suited to genome-wide studies of promoter interactions with distal regulatory elements, such as enhancers. We present the principles and methods for Promoter Capture Hi-C (PCHi-C), from experimental design to data analysis.
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hal-03708078 , version 1 (28-06-2022)

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Nezih Karasu, Thomas Sexton. Assessment of 3D Interactions Between Promoters and Distal Regulatory Elements with Promoter Capture Hi-C (PCHi-C). Methods in Molecular Biology, 2021, 2351, pp.229-248. ⟨10.1007/978-1-0716-1597-3_13⟩. ⟨hal-03708078⟩
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