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Article Dans Une Revue Journal of Investigative Dermatology Année : 1996

Cell Survival and Shuttle Vector Mutagenesis Induced by Ultraviolet A and Ultraviolet B Radiation in a Human Cell Line

Résumé

Although it is known that sunlight is carcinogenic,few molecular data are available concerning the mutagenic effects of ultraviolet (UV) B (290-320 nm) and UVA (320-400 nm) radiation in human cells. To analyze the biologic effects of UVA and UVB, we irradiated the 293 human cell line, derived from adenovirus-transformed human embryonic kidney cells, in which we had stably introduced a shuttle vector harboring the lacZ' bacterial gene as the mutagenesis target. Identical cell survival occurred after UVA doses 700-fold higher than UVB. This comparable to the UVA/UVB ratio that reaches the basal cell layer of the skin after sunlight exposure with UVB sunscreen. The frequency of UVA- and UVB- induced mutations increased with the UV dose as cell survival decreased. At cell survival levels greater than 10%, UVA and UVB induced similar frequencies of mutations in the episomal lacZ gene, whereas for cell survival lower than 10%, UVA induced twice as many mutations as UVB. Sequence analysis of 81 independent lacZ mutants (36 UVA- and 45 UVB-induced) revealed specific characteristics for some UV-induced-mutations, particularly for UVB. Mutations at A/T base pairs were induced more frequently by UVA than by UVB. The UVA-induced mutation spectrum that we have observed in human cells may help help to elucidate the mechanism of skin carcinogenesis.

Dates et versions

hal-03680157 , version 1 (06-07-2022)

Identifiants

Citer

Caroline Robert, Bernard Muel, Annie Benoit, Alain Sarasin, Louis Dubertret, et al.. Cell Survival and Shuttle Vector Mutagenesis Induced by Ultraviolet A and Ultraviolet B Radiation in a Human Cell Line. Journal of Investigative Dermatology, 1996, 106 (4), pp.721-728. ⟨10.1111/1523-1747.ep12345616⟩. ⟨hal-03680157⟩

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