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Journal Articles European Physical Journal E: Soft matter and biological physics Year : 2022

Measuring the average cell size and width of its distribution in cellular tissues using Fourier transform

Tess Homan
  • Function : Author
Sylvain Monnier
Cécile Jebane
  • Function : Author
Hélène Delanoë-Ayari

Abstract

We present an in-depth investigation of a fully automated Fourier-based analysis to determine the cell size and the width of its distribution in 3D biological tissues. The results are thoroughly tested using generated images, and we offer valuable criteria for image acquisition settings to optimize accuracy. We demonstrate that the most important parameter is the number of cells in the field of view, and we show that accurate measurements can already be made on volume only containing 3×3×3 cells. The resolution in z is also not so important, and a reduced number of in-depth images, of order of one per cell, already provides a measure of the mean cell size with less than 5% error. The technique thus appears to be a very promising tool for very fast live local volume cell measurement in 3D tissues in vivo while strongly limiting photobleaching and phototoxicity issues.

Dates and versions

hal-03670998 , version 1 (18-05-2022)

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Cite

Tess Homan, Sylvain Monnier, Cécile Jebane, Alice Nicolas, Hélène Delanoë-Ayari. Measuring the average cell size and width of its distribution in cellular tissues using Fourier transform. European Physical Journal E: Soft matter and biological physics, 2022, 45 (5), pp.44. ⟨10.1140/epje/s10189-022-00198-y⟩. ⟨hal-03670998⟩
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