Article Dans Une Revue Arteriosclerosis, Thrombosis, and Vascular Biology Année : 2018

Hypoalphalipoproteinemia and BRAF V600E Mutation Are Major Predictors of Aortic Infiltration in the Erdheim-Chester Disease

Lucie Poupel
  • Fonction : Auteur
Philippe Cluzel
Flora Saint-Charles
  • Fonction : Auteur
Frédéric Charlotte
Youssef Arsafi
  • Fonction : Auteur
Jean-François Emile
Jean Donadieu
  • Fonction : Auteur
Zahir Amoura
Julien Haroche
  • Fonction : Auteur

Résumé

Objective— Erdheim-Chester disease (ECD) is a rare non-Langerhans cell histiocytosis characterized by the infiltration of multiple tissues with lipid-laden histiocytes. Cardiovascular involvement is frequent in ECD and leads to a severe prognosis. The objective of this study was to determine whether an alteration of lipid metabolism participates in the lipid accumulation in histiocytes and the cardiovascular involvement in ECD. Approach and Results— An analysis of plasma lipid levels indicated that male ECD patients carrying the BRAF V600E (B-Raf proto-oncogene, serine/threonine kinase) mutation exhibited hypoalphalipoproteinemia, as demonstrated by low plasma HDL-C (high-density lipoprotein cholesterol) levels. Capacity of sera from male BRAF V600E ECD patients to mediate free cholesterol efflux from human macrophages was reduced compared with control individuals. Cardiovascular involvement was detected in 84% of the ECD patients, and we reported that the presence of the BRAF V600E mutation and hypoalphalipoproteinemia is an independent determinant of aortic infiltration in ECD. Phenotyping of blood CD14 + cells, the precursors of histiocytes, enabled the identification of a specific inflammatory signature associated with aortic infiltration which was partially affected by the HDL phenotype. Finally, the treatment with vemurafenib, an inhibitor of the BRAF V600E mutation, restored the defective sera cholesterol efflux capacity and reduced the aortic infiltration. Conclusions— Our findings indicate that hypoalphalipoproteinemia in male ECD patients carrying the BRAF V600E mutation favors the formation of lipid-laden histiocytes. In addition, we identified the BRAF status and the HDL phenotype as independent determinants of the aortic involvement in ECD with a potential role of HDL in modulating the infiltration of blood CD14 + cells into the aorta.

Dates et versions

hal-03625003 , version 1 (30-03-2022)

Identifiants

Citer

Fleur Cohen-Aubart, Maryse Guerin, Lucie Poupel, Philippe Cluzel, Flora Saint-Charles, et al.. Hypoalphalipoproteinemia and BRAF V600E Mutation Are Major Predictors of Aortic Infiltration in the Erdheim-Chester Disease. Arteriosclerosis, Thrombosis, and Vascular Biology, 2018, 38 (8), pp.1913-1925. ⟨10.1161/ATVBAHA.118.310803⟩. ⟨hal-03625003⟩
52 Consultations
0 Téléchargements

Altmetric

Partager

  • More