The TonB-dependent ferrichrome receptor FcuA of Yersinia enterocolitica: evidence against a strict co-evolution of receptor structure and substrate specificity - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Molecular Microbiology Année : 1993

The TonB-dependent ferrichrome receptor FcuA of Yersinia enterocolitica: evidence against a strict co-evolution of receptor structure and substrate specificity

Résumé

A Yersinia enterocolitica receptor mutant was isolated which is impaired in ferrichrome uptake. The receptor-encoding gene fcuA was cloned in Escherichia coli K-12. A fcuA mutant of Y. enterocolitica could be complemented by the cloned DNA fragment. The FcuA-encoding region was sequenced and an open reading frame encoding 758 amino acids including a signal sequence of 36 amino acids was found. FcuA shared 34.6% amino acid sequence homology with FatA, the anguibactin receptor of Vibrio anguiiiarum, but only 20.6% homology with FhuA, the ferrichrome receptor of E. coli. Since the structure of anguibactin differs strongly from that of ferrichrome there seems to be no co-evolution of receptor structure and substrate specificity. The ferrichromer eceptors FcuA from Y. enterocolitica and FhuA from E. coii had slightly different substrate specificities. In contrast to FhuA from E. coli, FcuA from Y. enterocolitica was more stereoselective and failed to transport enantio ferrichrome. Three additional ferrichrome receptors were cloned from Pantoea aggiomerans (formerly Erwinia herbicola), Saimoneiia paratyphi B and Saimoneiia typhimurium. Their substrate specificity was similar but not identical.
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hal-03620625 , version 1 (26-03-2022)

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Ralf Koebnik, Klaus Hantke, Volkmar Braun, Andreas Bäumler, Jürgen Heesemann. The TonB-dependent ferrichrome receptor FcuA of Yersinia enterocolitica: evidence against a strict co-evolution of receptor structure and substrate specificity. Molecular Microbiology, 1993, 7 (3), pp.383-393. ⟨10.1111/j.1365-2958.1993.tb01130.x⟩. ⟨hal-03620625⟩
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