An Integrated Multiple Electrochemical miRNA Sensing System Embedded into a Microfluidic Chip - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Biosensors Année : 2022

An Integrated Multiple Electrochemical miRNA Sensing System Embedded into a Microfluidic Chip

Résumé

In this article, we present the design, fabrication and characterization of a microfluidic device and a dedicated electronic system to perform 8 multiplexed electrochemical measurements of synthetic miRNA strands, as well as the biochemical protocols developed for the functionalization of the electrodes and the quantification experiments. The outcomes of this work highlight that the parallelization of eight microchannels containing 2-electrode cells driven by the dedicated electronics offers a solution as robust as a conventional 3-electrode cell and commercially available potentiostats. In addition, this solution presents the advantage of simultaneously reduce the microfabrication complexity, as well as offering an integrated; multiplexed and portable system for the quantification of miRNA. The results presented demonstrate that the system shows a linear response on concentrations down to 10−18 mol/L of perfect matched reporter and capture sequences of synthetic miRNA.
Fichier principal
Vignette du fichier
63-Losada et al-biosensors-12-00145.pdf (1.84 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03600731 , version 1 (07-03-2022)

Identifiants

Citer

Pedro Gonzalez-Losada, Martina Freisa, Claire Poujouly, Jean Gamby. An Integrated Multiple Electrochemical miRNA Sensing System Embedded into a Microfluidic Chip. Biosensors, 2022, 12 (3), pp.145. ⟨10.3390/bios12030145⟩. ⟨hal-03600731⟩
18 Consultations
31 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More