Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato - Archive ouverte HAL Access content directly
Journal Articles Plant Science Year : 2008

Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato

Abstract

Transcriptional co-activators of the Multiprotein Bridging Factor1 (MBF1) type belong to a multigenic family that encode key components of the machinery controlling gene expression by communicating between transcription factors and the basal transcription machinery. Knocking-down the expression of one member of the family has proved difficult probably due to functional redundancy. We show here that a fusion of SlER24, an MBF1 type gene of tomato, to the Ethylene-responsive element-binding associated Amphiphilic Repression (EAR) motif is capable of slowing down significantly the expression of the GFP protein driven by a synthetic ethylene-responsive GCC-rich promoter in a single cell transient expression system. A fusion of AtMBF1c of Arabidopsis to EAR, driven by the 35S promoter, caused a reduction of the percentage of seed germination and dwarfism of the plant. Similar fusion with the SlER24 of tomato in the MicroTom cultivar induced a delay of seed germination and no obvious effect on plant growth. Besides giving information on the role of the MBF1 genes in plant development, this study demonstrates that the EAR strategy is efficient not only for regular transcription factors as demonstrated so far, but also in the case of co-activators known to not bind directly to DNA.
Fichier principal
Vignette du fichier
Hommel_1731.pdf (1.15 Mo) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03591036 , version 1 (28-02-2022)

Identifiers

Cite

Marie Hommel, Qaiser Khalil Ahmad, Fabiola Jaimes Miranda, Isabelle Mila, Cécile Pouzet, et al.. Over-expression of a chimeric gene of the transcriptional co-activator MBF1 fused to the EAR repressor motif causes developmental alteration in Arabidopsis and tomato. Plant Science, 2008, 1 (1-2), pp.168-177. ⟨10.1016/j.plantsci.2008.01.019⟩. ⟨hal-03591036⟩
6 View
14 Download

Altmetric

Share

Gmail Facebook X LinkedIn More