The Fucoxanthin Chlorophyll a/c-Binding Protein in Tisochrysis lutea: Influence of Nitrogen and Light on Fucoxanthin and Chlorophyll a/c-Binding Protein Gene Expression and Fucoxanthin Synthesis
Résumé
We observed differences in classification in Chromista. We proposed a classification of the family with two groups specific to haptophytes, one specific to diatoms, and one specific to seaweeds. Identification and characterization of the Fucoxanthin and Chlorophyll -binding Protein (FCP) of the haptophyte microalgae were performed by similarity analysis. The FCP family contains 52 genes in . FCP pigment binding site candidates were characterized on Lhcf protein monomers of , which possesses at least nine chlorophylls and five fucoxanthin molecules, on average, per monomer. The expression of genes was assessed during turbidostat and chemostat experiments, one with constant light (CL) and changing nitrogen phases, the second with a 12 h:12 h sinusoidal photoperiod and changing nitrogen phases. RNA-seq analysis revealed a dynamic decrease in the expression of genes with nitrogen depletion. We observed that was only expressed at night, suggesting that its role is to protect \cells from return of light after prolonged darkness exposure.
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