A guanosine tetraphosphate (ppGpp) mediated brake on photosynthesis is required for acclimation to nitrogen limitation in Arabidopsis
Shanna Romand
(1)
,
Hela Abdelkefi
(1, 2)
,
Cécile Lecampion
(1)
,
Mohamed Belaroussi
(1)
,
Melanie Dussenne
(1)
,
Brigitte Ksas
(3)
,
Sylvie Citerne
(4)
,
Jose Caius
(5)
,
Stefano d'Alessandro
(1)
,
Hatem Fakhfakh
(2, 6)
,
Stefano Caffarri
(1)
,
Michel Havaux
(3)
,
Benjamin Field
(1)
1
LGBP -
Luminy Génétique et Biophysique des Plantes
2 University of Tunis El Manar
3 PEPSS - Plant Environmental Physiology and Stress Signaling
4 IJPB - Institut Jean-Pierre Bourgin
5 IPS2 (UMR_9213 / UMR_1403) - Institut des Sciences des Plantes de Paris-Saclay
6 UCAR - Université de Carthage (Tunisie)
2 University of Tunis El Manar
3 PEPSS - Plant Environmental Physiology and Stress Signaling
4 IJPB - Institut Jean-Pierre Bourgin
5 IPS2 (UMR_9213 / UMR_1403) - Institut des Sciences des Plantes de Paris-Saclay
6 UCAR - Université de Carthage (Tunisie)
Cécile Lecampion
- Fonction : Auteur
- PersonId : 1370106
- IdHAL : cecile-lecampion
- ORCID : 0000-0002-7862-517X
Brigitte Ksas
- Fonction : Auteur
- PersonId : 759902
- ORCID : 0000-0002-5369-3176
Jose Caius
- Fonction : Auteur
- PersonId : 1320220
- ORCID : 0000-0001-8692-042X
Michel Havaux
- Fonction : Auteur
- PersonId : 821050
- ORCID : 0000-0002-6434-393X
- IdRef : 176232745
Benjamin Field
- Fonction : Auteur
- PersonId : 739690
- IdHAL : ben-field
- ORCID : 0000-0003-2142-4606
- IdRef : 22369715X
Résumé
Guanosine pentaphosphate and tetraphosphate (together referred to as ppGpp) are hyperphosphorylated nucleotides found in bacteria and the chloroplasts of plants and algae. In plants and algae artificial ppGpp accumulation can inhibit chloroplast gene expression, and influence photosynthesis, nutrient remobilisation, growth, and immunity. However, it is so far unknown whether ppGpp is required for abiotic stress acclimation in plants. Here, we demonstrate that ppGpp biosynthesis is necessary for acclimation to nitrogen starvation in Arabidopsis. We show that ppGpp is required for remodeling the photosynthetic electron transport chain to downregulate photosynthetic activity and for protection against oxidative stress. Furthermore, we demonstrate that ppGpp is required for coupling chloroplastic and nuclear gene expression during nitrogen starvation. Altogether, our work indicates that ppGpp is a pivotal regulator of chloroplast activity for stress acclimation in plants.
Format du dépôt | Fichier |
---|---|
Type de dépôt | Article dans une revue |
Titre |
en
A guanosine tetraphosphate (ppGpp) mediated brake on photosynthesis is required for acclimation to nitrogen limitation in Arabidopsis
|
Résumé |
en
Guanosine pentaphosphate and tetraphosphate (together referred to as ppGpp) are hyperphosphorylated nucleotides found in bacteria and the chloroplasts of plants and algae. In plants and algae artificial ppGpp accumulation can inhibit chloroplast gene expression, and influence photosynthesis, nutrient remobilisation, growth, and immunity. However, it is so far unknown whether ppGpp is required for abiotic stress acclimation in plants. Here, we demonstrate that ppGpp biosynthesis is necessary for acclimation to nitrogen starvation in Arabidopsis. We show that ppGpp is required for remodeling the photosynthetic electron transport chain to downregulate photosynthetic activity and for protection against oxidative stress. Furthermore, we demonstrate that ppGpp is required for coupling chloroplastic and nuclear gene expression during nitrogen starvation. Altogether, our work indicates that ppGpp is a pivotal regulator of chloroplast activity for stress acclimation in plants.
|
Auteur(s) |
Shanna Romand
1
, Hela Abdelkefi
1, 2
, Cécile Lecampion
1
, Mohamed Belaroussi
1
, Melanie Dussenne
1
, Brigitte Ksas
3
, Sylvie Citerne
4
, Jose Caius
5
, Stefano d'Alessandro
1
, Hatem Fakhfakh
2, 6
, Stefano Caffarri
1
, Michel Havaux
3
, Benjamin Field
1
1
LGBP -
Luminy Génétique et Biophysique des Plantes
( 40431 )
- ex-Laboratoire de Génétique et Biophysique des Plantes
- France
2
University of Tunis El Manar
( 1061809 )
- Université de Tunis El Manar : Campus Universitaire Farhat Hached B.P. n° 94 - ROMMANA 1068 Tunis, Tunisie
- Tunisie
3
PEPSS -
Plant Environmental Physiology and Stress Signaling
( 40424 )
- ex-SAVE (Signalisation de l'Adaptation des Végétaux à l'Environnement)
ex-LBDP (Laboratoire de Biologie du Développement des Plantes)
- France
4
IJPB -
Institut Jean-Pierre Bourgin
( 1001923 )
- INRAE Centre de Versailles-Grignon, Route de St-Cyr (RD10), 78026 Versailles Cedex
- France
5
IPS2 (UMR_9213 / UMR_1403) -
Institut des Sciences des Plantes de Paris-Saclay
( 1002472 )
- Institute of Plant Sciences - Paris-Saclay
Bâtiment 630, rue de Noetzlin
Plateau du Moulon
CS80004
91192 - Gif-sur-Yvette Cedex
- France
6
UCAR -
Université de Carthage (Tunisie)
( 232921 )
- Avenue de la République, BP 77 - 1054 Amilcar
- Tunisie
|
Public visé |
Scientifique
|
Langue du document |
Anglais
|
Licence |
Paternité
|
Nom de la revue |
|
Vulgarisation |
Non
|
Comité de lecture |
Oui
|
Audience |
Internationale
|
Date de publication |
2022-02-14
|
Volume |
11
|
Domaine(s) |
|
Projet(s) ANR |
|
Mots-clés |
en
A. thaliana, chloroplast, nitrogen, photoprotection, photosynthesis, plant biology, ppGpp
|
DOI | 10.7554/eLife.75041 |
Pubmed Id | 35156611 |
Origine :
Fichiers produits par l'(les) auteur(s)
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