In vivo spatiotemporal control of voltage-gated ion channels by using photoactivatable peptidic toxins - Archive ouverte HAL
Article Dans Une Revue Nature Communications Année : 2022

In vivo spatiotemporal control of voltage-gated ion channels by using photoactivatable peptidic toxins

Herve Meudal
Aude Lafoux
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Rémy Béroud
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Chris Jopling
Corinne Huchet
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Céline Landon

Résumé

Abstract Photoactivatable drugs targeting ligand-gated ion channels open up new opportunities for light-guided therapeutic interventions. Photoactivable toxins targeting ion channels have the potential to control excitable cell activities with low invasiveness and high spatiotemporal precision. As proof-of-concept, we develop HwTxIV-Nvoc, a UV light-cleavable and photoactivatable peptide that targets voltage-gated sodium (Na V ) channels and validate its activity in vitro in HEK293 cells, ex vivo in brain slices and in vivo on mice neuromuscular junctions. We find that HwTxIV-Nvoc enables precise spatiotemporal control of neuronal Na V channel function under all conditions tested. By creating multiple photoactivatable toxins, we demonstrate the broad applicability of this toxin-photoactivation technology.
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hal-03578128 , version 1 (17-02-2022)

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Jérôme Montnach, Laila Ananda Blömer, Ludivine Lopez, Luiza Filipis, Herve Meudal, et al.. In vivo spatiotemporal control of voltage-gated ion channels by using photoactivatable peptidic toxins. Nature Communications, 2022, 13 (1), pp.417. ⟨10.1038/s41467-022-27974-w⟩. ⟨hal-03578128⟩
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