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Article Dans Une Revue ChemMedChem Année : 2016

Quantitative MALDI‐MS Binding Assays: An Alternative to Radiolabeling

Résumé

Radiolabeling of ligands is still the gold standard in the study of high-affinity receptor-ligand interactions. In an effort toward safer and simpler alternatives to the use of radioisotopes, we developed a quantitative and highly sensitive matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) method that relies on the use of chemically tagged ligands designed to be specifically detectable when present as traces in complex biological mixtures such as cellular lysates. This innovative technology allows easy, sensitive detection and accurate quantification of analytes at the sub-nanomolar level. After statistical validation, we were able to perform pharmacological evaluations of G protein-coupled receptor (V1A-R)-ligand interactions. Both saturation and competitive binding assays were successfully processed.

Dates et versions

hal-03562144 , version 1 (08-02-2022)

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Maxime Rossato, Guillaume Miralles, Céline M'Kadmi, Mathieu Maingot, Muriel Amblard, et al.. Quantitative MALDI‐MS Binding Assays: An Alternative to Radiolabeling. ChemMedChem, 2016, 11 (23), pp.2582-2587. ⟨10.1002/cmdc.201600447⟩. ⟨hal-03562144⟩
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