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Article Dans Une Revue Bioelectrochemistry Année : 2017

A rapid and simple protocol to prepare a living biocomposite that mimics electroactive biofilms

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A living material was formed by self-assembly of bacterial cells (Shewanella oneidensis MR-1 or Pseudomonas fluorescens) with carbon nanotubes in the presence of cytochrome c from a bovine heart with the goal to mimic electroactive biofilms. The role of cytochrome c on self-assembly, cell viability and extracellular electron transfer was studied. Scanning electron microscopy and dynamic light scattering experiments highlighted its role on the self-assembly of bacteria-carbon nanotube aggregates within only 2 hours in solution. The deposition of these aggregates on glassy carbon surfaces led to a homogenous composite film in which the bacteria were embedded in a carbon nanotube network. A comparable cell density of 1 cell µm-2 was achieved in the presence or in the absence of cytochrome c, but this protein allowed maintaining a higher bacterial viability. Electrochemical characterization demonstrated the role of cytochrome c on electron transfer reactions, leading to a current density of up to 300 µA cm-2 in the presence of 50 mM formate when a porous carbon felt electrode is used as support for the biocomposite
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hal-03561994 , version 1 (08-02-2022)

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Stéphane Pinck, Mathieu Etienne, Manuel Dossot, Frédéric P.A. Jorand. A rapid and simple protocol to prepare a living biocomposite that mimics electroactive biofilms. Bioelectrochemistry, 2017, 118, pp.131-138. ⟨10.1016/j.bioelechem.2017.07.010⟩. ⟨hal-03561994⟩
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