Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Clinical Oral Investigations Année : 2020

Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation

Résumé

Introduction Endodontic treatment success is achieved not only when the cement provides a hermetic seal but also when the injured periapical tissue is regenerated. However, an exaggerated inflammatory reaction hinders tissue regeneration and it has been shown that dental materials affect the inflammatory response through modulation of cytokine secretion. This work was set to investigate the effects of the presence of hydrocortisone in zinc oxide eugenol sealers (Endomethasone N) on modulating the initial steps of inflammation in vitro. Material and methods Hydrocortisone and eugenol leaching from Endomethasone N and Pulp Canal Sealer (PCS) were quantified by ELISA and spectrofluorometry, respectively. The effects of Endomethasone N and Pulp Canal Sealer were studied on lipopolysaccharides (LPS)-stimulated human periodontal ligament (hPDL) cells. Cytokine (IL-6, TNF-α) secretion from cells was quantified by ELISA. Inflammatory cell (THP-1) adhesion to activated endothelial cells, their migration and activation were studied in vitro. Results Endomethasone N decreased secretion of IL-6 and TNF-α from hPDL cells. THP-1 adhesion to activated endothelial cells (HUVECs) and migration significantly decreased with Endomethasone N while no effect was observed with PCS. Activation of THP-1 decreased with both materials’ extracts but was significantly lower with Endomethasone N than with PCS. Conclusion These results performed in vitro show that Endomethasone N anti-inflammatory effects are due to the presence of hydrocortisone. Clinical relevance Endomethasone N has potential local anti-inflammatory effects which appear to be due to its hydrocortisone rather than eugenol content. Decreasing the inflammatory response is a pre-requisite to initiate the periapical healing.
Fichier principal
Vignette du fichier
Jeanneau et al. - 2020 - Investigating unset endodontic sealersÔÇÖ eugenol an-Copier.pdf (700.37 Ko) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03547442 , version 1 (05-02-2024)

Identifiants

Citer

Charlotte Jeanneau, Thomas Giraud, Jean-Louis Milan, Imad About. Investigating unset endodontic sealers’ eugenol and hydrocortisone roles in modulating the initial steps of inflammation. Clinical Oral Investigations, 2020, 24 (2), pp.639-647. ⟨10.1007/s00784-019-02957-2⟩. ⟨hal-03547442⟩
11 Consultations
4 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More