C5L2 Silencing in Human Pulp Fibroblasts Enhances Nerve Outgrowth Under Lipoteichoic Acid Stimulation - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Endodontics Année : 2018

C5L2 Silencing in Human Pulp Fibroblasts Enhances Nerve Outgrowth Under Lipoteichoic Acid Stimulation

Fanny Chmilewsky
  • Fonction : Auteur
Lyndon F Cooper
  • Fonction : Auteur
Seung H Chung
  • Fonction : Auteur

Résumé

Introduction: We recently reported that cariesassociated C5a receptor (C5aR) expression and activation result in up-regulation of brain-derived neurotropic factor secretion by pulp fibroblasts inducing prominent neurite outgrowth toward the carious site. Our data further showed a negative regulation of this brainderived neurotropic factor secretion by C5L2, another C5aR. C5L2 was considered a nonfunctional receptor and thus has received much less attention than C5aR. The aim of this study was to identify the role of C5L2 in pulp fibroblast-mediated neurite outgrowth. Methods: In this study, lipoteichoic acid (LTA) was used to mimic dental caries-like inflammation. To evaluate the role of C5L2 in pulp neurite outgrowth, human pulp fibroblasts were C5L2 small interfering RNA silenced and cocultured with human neurons in a nerve growth assay system. Results: C5L2 silencing drastically increased the neurite outgrowth toward the LTAstimulated pulp fibroblasts. The number of neurites detected was increased in the LTA-treated pulp fibroblasts. Conclusions: Our results show that C5L2 constitutes a negative regulator of the neurite outgrowth under LTA stimulation. Of the events occurring during dentin-pulp regeneration, nerve regeneration is the key factor for maintaining tooth viability after infection or injury. Our study provides a foundation for creating therapeutic tools that target pulp fibroblasts during pulp/nerve regeneration.
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Dates et versions

hal-03547385 , version 1 (28-01-2022)

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Fanny Chmilewsky, Imad About, Lyndon F Cooper, Seung H Chung. C5L2 Silencing in Human Pulp Fibroblasts Enhances Nerve Outgrowth Under Lipoteichoic Acid Stimulation. Journal of Endodontics, 2018, 44 (9), pp.1396-1401. ⟨10.1016/j.joen.2018.05.004⟩. ⟨hal-03547385⟩
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