Experimental setup for optical observation of ultrasound-assisted cell manipulation
Résumé
Ultrasonic technology is available, affordable and non-invasive. We use ultrasound to manipulate biological cells and micromaterials. The ultimate purpose of ultrasonic cell manipulation is the separation and eradication of unwanted cells.
This study presents and experimental setup designed for ultrasonic cell manipulation.
A perspex water-filled container is placed on the microscope stage of an Olympus CKX31 inverted microscope (Olympus Corporation, Shinjuku, Tokyo, Japan) with an Olympus CX PL C-Plan 20× objective lens (Olympus Corporation, Shinjuku, Tokyo, Japan). The objective has a numerical aperture of 0.4 and a working distance of 1.2 mm. The illumination is from the top.
The charge coupled device of a FASTCAM MC1 high-speed camera (Photron (Europe) Limited, West Wycombe, Bucks, United Kingdom) is mounted on the microscopic eyepiece through an adapter and connected to its processing unit. The camera is controlled by a laptop computer.
A cellulose capillary with a 200 µm internal diameter is fixed across the intersection of the optical and acoustic focus. Biological cells flow through the capillary. These cells are sonicated with an ultrasound transducer, mounted at variable angles.
This setup enables the study of cellular dynamics with an optical resolution below 1 µm and interframe times below 1 millisecond.
Domaines
Physique Médicale [physics.med-ph]
Origine : Fichiers produits par l'(les) auteur(s)