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Poster De Conférence Année : 2022

Implementation of new methods for metagenomics analysis using PacBio HiFi sequencing

Résumé

The variable regions of 16S rRNA gene is widely used to profile microbial communities, however the limited read lengths (e.g 460pb for the V3-V4 region) combined with the complexity of microbial samples makes it difficult to accurately identify bacterial strains and their abundance. The arrival PacBio Sequel II sequencer allow us to generate longer reads named "HiFi reads" using the circular consensus sequencing (CCS) mode, with an accuracy similar to the one observed for short reads sequencers.
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Dates et versions

hal-03541346 , version 1 (24-01-2022)

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  • HAL Id : hal-03541346 , version 1

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Adrien Castinel, Jean Mainguy, Sylvie Combes, Carole Iampietro, Christine Gaspin, et al.. Implementation of new methods for metagenomics analysis using PacBio HiFi sequencing. Plant & Animal Genome Conference (PAG) 2022, Jan 2022, San Diego, United States. ⟨hal-03541346⟩

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