Rationale Producing robust high-frequency time series of raw atmospheric water vapor isotope data using laser spectrometry requires accurate calibration. In particular, the chemical composition of the analyzed sample gas can cause isotope bias. This study assesses the matrix effect on calibrated delta O-17, delta O-18, delta H-2, O-17-excess, and d-excess values of atmospheric water vapor. Methods A Picarro L2140-i cavity ring-down spectrometer with an autosampler and a vaporizer is used to analyze delta O-17, delta O-18, delta H-2, O-17-excess, and d-excess of two water standards. Isotope data obtained using synthetic air and dry ambient air as carrier gas at water mixing ratios ranging from 2000 to 30 000 ppmv are compared. Based on the results, atmospheric water vapor measurements are calibrated. The expected precision is estimated by Monte Carlo simulation. Results The dry air source strongly impacts raw isotope values of the two water standards but has no effect on the mixing ratio dependency functions. When synthetic air is used, delta O-17, delta O-18, and O-17-excess of calibrated atmospheric water vapor are overestimated by 0.6 parts per thousand, 0.7 parts per thousand, and 217 per meg, respectively, whereas delta H-2 and d-excess are underestimated by 1.5 parts per thousand and 7.3 parts per thousand. Optimum precisions for the calibrated delta O-17, delta O-18, delta H-2, O-17-excess, and d-excess values and 12 min integration time are 0.02 parts per thousand, 0.03 parts per thousand, 0.4 parts per thousand, 14 per meg, and 0.4 parts per thousand, respectively. Conclusions Regarding the obtained results, recommendations for the calibration of atmospheric water vapor isotope measurements are presented. The necessity to use dry ambient air as dry air source when running the standards for calibration is pointed out as a prerequisite for accurate atmospheric water vapor O-17-excess and d-excess measurements