Proof of the resolution-doubling of Random Illumination Microscopy using the variance of the speckled images - Archive ouverte HAL
Communication Dans Un Congrès Année : 2021

Proof of the resolution-doubling of Random Illumination Microscopy using the variance of the speckled images

Anne Sentenac
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Marc Allain
Thomas Mangeat

Résumé

Owing to minor modifications of the optical setup, Random Illumination Microscopy (RIM) surpasses the resolution limit of a standard fluorescence microscope. RIM uses speckle illuminations of the sample to derive a single variance image from the resulting diffraction-limited acquisitions. Variancematching iterations then produce a super-resolved estimate of the sample. Here, we demonstrate that in the noiseless case, variancematching yields a unique solution for the set of spatial frequencies corresponding to a doubled resolution limit. A similar result was already proven for covariance-matching, but covariancebased iterations are not implementable in practice, due to the huge size of the covariance matrix and to the induced numerical complexity. Our new identifiability result is a strong theoretical evidence supporting the super-resolution capability of the variance-matching version of RIM.
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Dates et versions

hal-03538553 , version 1 (21-01-2022)

Identifiants

Citer

Simon Labouesse, Jérôme Idier, Anne Sentenac, Marc Allain, Thomas Mangeat. Proof of the resolution-doubling of Random Illumination Microscopy using the variance of the speckled images. 2021 29th European Signal Processing Conference (EUSIPCO), Aug 2021, Dublin, Ireland. pp.1159-1162, ⟨10.23919/EUSIPCO54536.2021.9616038⟩. ⟨hal-03538553⟩
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