Metabolic profiling strategy for determination of the absorption peak of artichoke leaf extract in human enriched serum - Archive ouverte HAL Accéder directement au contenu
Communication Dans Un Congrès Année : 2021

Metabolic profiling strategy for determination of the absorption peak of artichoke leaf extract in human enriched serum

Résumé

Our society nowadays is aging and the chronic disease appearance is increasing. To prevent this, plant-derived nutrients like polyphenols could be used. They are known to protect tissues from inflammatory and metabolic dysregulation induced by chronic diseases. Polyphenols are found in high level in artichoke leaf extract (ALE) and his ingestion may be effective to prevent such dysregulations. To prove this, an innovative clinical approach was designed. Patients ingested ALE and their blood was collected every 20 min after the ingestion during 240 min. The kinetic profile of the ALE absorption was studied by 1H NMR spectroscopy. The comparison of 1D spectra showed that the highest variations were observed in the phenolic compounds’ region and for the 100 min samples. The addition of the degradation products of chlorogenic acid, the major ALE polyphenol compound, to a naive serum led to similar chemical shift variations to those observed for the 100 min samples suggesting that the metabolic changes in the serum profile after ingestion were due to the presence of the ALE polyphenol metabolites. These results, then, allowed to continue the clinical trial and to determine the influence of sera enriched in metabolites resulting from ALE consumption on the behavior of human hepatocyte, adipocyte and chondrocyte cultures. Altogether, the obtained data show that polyphenols from artichoke may represent a relevant alternative for nutritional strategies addressing chronic disease issue [1]. References: 1. Wauquier F.; Boutin-Wittrant L.; Viret A.; Guilhaudis L et al. Nutrients. 2021, 13 (8), 2653. DOI: https://doi.org/10.3390/nu13082653
Fichier non déposé

Dates et versions

hal-03529874 , version 1 (17-01-2022)

Identifiants

  • HAL Id : hal-03529874 , version 1

Citer

Aurélien Viret, Asma Bourafai-Aziez, Fabien F. Wauquier, Line Boutin-Wittrant, Glwadys Charpentier, et al.. Metabolic profiling strategy for determination of the absorption peak of artichoke leaf extract in human enriched serum. 14ème journées scientifiques du RFMF, Nov 2021, Aussois, France. ⟨hal-03529874⟩
64 Consultations
0 Téléchargements

Partager

Gmail Facebook X LinkedIn More