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Article Dans Une Revue Methods in Molecular Biology Année : 2022

The High-salt Recovered Sequence-sequencing (HRS-seq) method: exploring genome association with nuclear bodies

Résumé

Recent works indicate that, at specific loci, interactions of chromatin with membrane-less organelles self-assembled through mechanisms of phase separation, like nuclear bodies, are crucial to regulate genome functions, and in particular transcription. Here we describe the protocol of the High-salt Recovered Sequence sequencing method whose principle relies on high-throughput sequencing of genomic DNA trapped into large RNP complexes that are made insoluble by high-salt treatments.
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Dates et versions

hal-03500289 , version 1 (22-12-2021)

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Citer

Cosette Rebouissou, Marie-Odile Baudement, Thierry Forné. The High-salt Recovered Sequence-sequencing (HRS-seq) method: exploring genome association with nuclear bodies. Methods in Molecular Biology, 2022, Spatial Genome Organization: Methods and Protocols, 2532, pp.187-197. ⟨10.1007/978-1-0716-2497-5_9⟩. ⟨hal-03500289⟩
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