Ethylene-regulated gene expression in tomato fruit: characterization of novel ethylene-responsive and ripeningrelated genes isolated by differential display. - Archive ouverte HAL Access content directly
Journal Articles Plant Journal Year : 1999

Ethylene-regulated gene expression in tomato fruit: characterization of novel ethylene-responsive and ripeningrelated genes isolated by differential display.

Hicham Zegzouti
  • Function : Author
Brian Jones
  • Function : Author
Pierre Frasse
  • Function : Author
Christel Marty
  • Function : Author
Béatrice Maitre
  • Function : Author
Mondher Bouzayen

Abstract

Differential display was used to isolate early ethyleneregulated genes from late immature green tomato fruit in order to obtain a broader understanding of the molecular basis by which ethylene coordinates the ripening process. Nineteen novel ethylene-responsive (ER) cDNA clones were isolated that fell into three classes: (i) ethylene up-regulated (ii) ethylene downregulated, and (iii) transiently induced. Expression analysis revealed that ethylene-dependent changes in mRNA accumulation occurred rapidly (15 min) for most of the ER clones. The predicted proteins encoded by the ER genes are putatively involved in processes as diverse as primary metabolism, hormone signalling and stress responses. Although a number of the isolated ER clones correspond to genes already documented in other species, their responsiveness to ethylene is described here for the ®rst time. Among the ER clones sharing high homology with regulatory genes, ER43, a putative GTP-binding protein, and ER50, a CTR1-like clone, are potentially involved in signal transduction. ER24 is homologous to the multiprotein bridging factor MBF1 involved in transcriptional activation, and ®nally, two clones are homologous to genes involved in post-transcriptional regulation: ER49, a putative translational elongation factor, and ER68, a mRNA helicase-like gene. Six ER clones correspond to as yet unidenti®ed genes. The expression studies indicated that all the ER genes are ripening-regulated, and, depending on the clone, show changes in transcript accumulation either at the breaker, turning, or red stage. Analysis of transcript accumulation in different organs indicated a strong bias towards expression in the fruit for many of the clones. The potential roles for some of the ER clones
Fichier principal
Vignette du fichier
Zegzouti_1385.pdf (482.27 Ko) Télécharger le fichier
Origin : Files produced by the author(s)

Dates and versions

hal-03484885 , version 1 (17-12-2021)

Identifiers

Cite

Hicham Zegzouti, Brian Jones, Pierre Frasse, Christel Marty, Béatrice Maitre, et al.. Ethylene-regulated gene expression in tomato fruit: characterization of novel ethylene-responsive and ripeningrelated genes isolated by differential display.. Plant Journal, 1999, 1 (6), pp.589-600. ⟨10.1046/j.1365-313x.1999.00483.x⟩. ⟨hal-03484885⟩
6 View
9 Download

Altmetric

Share

Gmail Facebook X LinkedIn More