ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development - Archive ouverte HAL Access content directly
Journal Articles Plant Journal Year : 2004

ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development

Abstract

Gene expression profiling holds tremendous promise for dissecting the regulatory mechanisms and transcriptional networks that underlie biological processes. Here we provide details of approaches used by others and ourselves for gene expression profiling in plants with emphasis on cDNA microarrays and discussion of both experimental design and downstream analysis. We focus on methods and techniques emphasizing fabrication of cDNA microarrays, fluorescent labeling, cDNA hybridization, experimental design, and data processing. We include specific examples that demonstrate how this technology can be used to further our understanding of plant physiology and development (specifically fruit development and ripening) and for comparative genomics by comparing transcriptome activity in tomato and pepper fruit.
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Dates and versions

hal-03483284 , version 1 (16-12-2021)

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Rob Alba, Zhangjun Fei, Paxton Payton, Yang Liu, Shanna L. Moore, et al.. ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development. Plant Journal, 2004, 3 (5), pp.697-714. ⟨10.1111/j.1365-313X.2004.02178.x⟩. ⟨hal-03483284⟩
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