ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue The Plant Journal Année : 2004

ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development

Résumé

Gene expression profiling holds tremendous promise for dissecting the regulatory mechanisms and transcriptional networks that underlie biological processes. Here we provide details of approaches used by others and ourselves for gene expression profiling in plants with emphasis on cDNA microarrays and discussion of both experimental design and downstream analysis. We focus on methods and techniques emphasizing fabrication of cDNA microarrays, fluorescent labeling, cDNA hybridization, experimental design, and data processing. We include specific examples that demonstrate how this technology can be used to further our understanding of plant physiology and development (specifically fruit development and ripening) and for comparative genomics by comparing transcriptome activity in tomato and pepper fruit.
Fichier principal
Vignette du fichier
Alba_1363.pdf (1.24 Mo) Télécharger le fichier
Origine Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03483284 , version 1 (16-12-2021)

Identifiants

Citer

Rob Alba, Zhangjun Fei, Paxton Payton, Yang Liu, Shanna L. Moore, et al.. ESTs, cDNA microarrays, and gene expression profiling : tools for dissecting plant physiology and development. The Plant Journal, 2004, 3 (5), pp.697-714. ⟨10.1111/j.1365-313X.2004.02178.x⟩. ⟨hal-03483284⟩
12 Consultations
137 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More