Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A - Archive ouverte HAL Access content directly
Journal Articles Molecular Nutrition & Food Research Year : 2006

Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A

Ali Atoui
  • Function : Author
Huy Phong Dao
  • Function : Author
Florence Mathieu

Abstract

The diversity of polyketide synthase (PKS) genes in Aspergillus ochraceus NRRL 3174 and Aspergillus carbonarius 2Mu134 has been investigated using different primer pairs previously developed for the ketosynthase (KS) domain of fungal PKSs. Nine different KS domain sequences in A. ochraceus NRRL 3174 as well as five different KS domain sequences in A. carbonarius 2Mu134 have been identified. The identified KS fragments were distributed in five different clusters on the phylogenetic tree, indicating that they most probably represent PKSs responsible for different functions.
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Dates and versions

hal-03482063 , version 1 (15-12-2021)

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Ali Atoui, Huy Phong Dao, Florence Mathieu, Ahmed Lebrihi. Amplification and diversity analysis of ketosynthase domains of putative polyketide synthase genes in Aspergillus ochraceus and Aspergillus carbonarius producers of ochratoxin A. Molecular Nutrition & Food Research, 2006, 5 (6), pp.488-493. ⟨10.1002/mnfr.200500165⟩. ⟨hal-03482063⟩
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