Development of bacterial resistance to antibiotics has lead to investigations of rare bacteria, which produce new bioactive molecules. Saccharothrix algriensis has been isolated from the desert Maghreb. It produces dithiolopyrrolones, some of which were newly identified. In order to optimize and control production of dithiolopyrrolones, investigation regarding microorganism metabolism was required. Growth on semisynthetic medium containing 2 g⋅ l–1 of yeast extract was complicated because it was performed on several substrates. Moreover, because development of this bacterium on minimum medium was difficult, its composition was optimized by screening of different compounds led by yeast extract. Uracil added to the minimum medium allowed a maximum biomass production of 1.35 g⋅ l–1 compared to 0.32 g⋅ l–1 without uracil. Moreover, nonpolar amino acids and trace metal elements stimulated Saccharothrix algeriensis growth.