Sps-3 transcript levels are determined by multiple remote sequence elements. - Archive ouverte HAL Access content directly
Journal Articles EMBO Journal Year : 1987

Sps-3 transcript levels are determined by multiple remote sequence elements.

Abstract

The region from 1.4 to 2.7 kb upstream of Drosophila melanogaster gene Sgs-3 is responsible for a 10-fold increase in Sgs-3 transcript levels in the third instar larval salivary gland. This region includes the related Sgs-7 gene from the 68C glue gene cluster as well as 400 bp of its 5' sequences. We show that two elements are involved, each contributing a modest 3-fold effect. One of these includes Sgs-7 transcribed sequences some 2.3 kb upstream of Sgs-3, although Sgs-7 transcription is not involved. Although important for the overall levels of Sgs-3 expression, they are clearly not strong, viral-like enhancer elements. We propose that many position effects observed in P element transformation studies are the consequence of insertion in the vicinity of similar elements dispersed throughout the genome and having modest effects on transcript levels.

Domains

Genetics

Dates and versions

hal-03475626 , version 1 (11-12-2021)

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Cite

A Giangrande, C Mettling, G Richards. Sps-3 transcript levels are determined by multiple remote sequence elements.. EMBO Journal, 1987, 6 (10), pp.3079-84. ⟨hal-03475626⟩
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