Proteomic Survey Reveals Altered Energetic Patterns and Metabolic Failure Prior to Retinal Degeneration - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Journal of Neuroscience Année : 2014

Proteomic Survey Reveals Altered Energetic Patterns and Metabolic Failure Prior to Retinal Degeneration

Ana Griciuc
  • Fonction : Auteur
Michel J Roux
Juliane Merl
  • Fonction : Auteur
Stefanie M Hauck
  • Fonction : Auteur
Liviu Aron
  • Fonction : Auteur
Marius Ueffing
  • Fonction : Auteur
  • PersonId : 909989

Résumé

Inherited mutations that lead to misfolding of the visual pigment rhodopsin (Rho) are a prominent cause of photoreceptor neuron (PN) degeneration and blindness. How Rho proteotoxic stress progressively impairs PN viability remains unknown. To identify the pathways that mediate Rho toxicity in PNs, we performed a comprehensive proteomic profiling of retinas from Drosophila transgenics expressing Rh1 P37H , the equivalent of mammalian Rho P23H , the most common Rho mutation linked to blindness in humans. Profiling of young Rh1 P37H retinas revealed a coordinated upregulation of energy-producing pathways and attenuation of energy-consuming pathways involving target of rapamycin (TOR) signaling, which was reversed in older retinas at the onset of PN degeneration. We probed the relevance of these metabolic changes to PN survival by using a combination of pharmacological and genetic approaches. Chronic suppression of TOR signaling, using the inhibitor rapamycin, strongly mitigated PN degeneration, indicating that TOR signaling activation by chronic Rh1 P37H proteotoxic stress is deleterious for PNs. Genetic inactivation of the endoplasmic reticulum stress-induced JNK/TRAF1 axis as well as the APAF-1/caspase-9 axis, activated by damaged mitochondria, dramatically suppressed Rh1 P37H-induced PN degeneration, identifying the mitochondria as novel mediators of Rh1 P37H toxicity. We thus propose that chronic Rh1 P37H proteotoxic stress distorts the energetic profile of PNs leading to metabolic imbalance, mitochondrial failure, and PN degeneration and therapies normalizing metabolic function might be used to alleviate Rh1 P37H toxicity in the retina. Our study offers a glimpse into the intricate higher order interactions that underlie PN dysfunction and provides a useful resource for identifying other molecular networks that mediate Rho toxicity in PNs.

Dates et versions

hal-03447891 , version 1 (15-12-2021)

Identifiants

Citer

Ana Griciuc, Michel J Roux, Juliane Merl, Angela Giangrande, Stefanie M Hauck, et al.. Proteomic Survey Reveals Altered Energetic Patterns and Metabolic Failure Prior to Retinal Degeneration. Journal of Neuroscience, 2014, 34 (8), pp.2797-2812. ⟨10.1523/JNEUROSCI.2982-13.2014⟩. ⟨hal-03447891⟩
55 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More