Pré-Publication, Document De Travail Année : 2021

The control of transcriptional memory by stable mitotic bookmarking

Maëlle Bellec
Jérémy Dufourt
George Hunt
Hélène Lenden-Hasse
  • Fonction : Auteur
Antonio Trullo
Amal Zine El Aabidine
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Marie Lamarque
Marissa Gaskill
Heloïse Faure-Gautron
  • Fonction : Auteur
Mattias Mannervik
Melissa Harrison
Jean-Christophe Andrau
Cyril Favard
Ovidiu Radulescu

Résumé

Abstract To maintain cellular identities during development, gene expression profiles must be faithfully propagated through cell generations. The reestablishment of gene expression patterns upon mitotic exit is thought to be mediated, in part, by mitotic bookmarking by transcription factors (TF). However, the mechanisms and functions of TF mitotic bookmarking during early embryogenesis remain poorly understood. In this study, taking advantage of the naturally synchronized mitoses of Drosophila early embryos, we provide evidence that the pioneer-like transcription factor GAF acts as stable mitotic bookmarker during zygotic genome activation. We report that GAF remains associated to a large fraction of its interphase targets including at cis -regulatory sequences of key developmental genes, with both active and repressive chromatin signatures. GAF mitotic targets are globally accessible during mitosis and are bookmarked via histone acetylation (H4K8ac). By monitoring the kinetics of transcriptional activation in living embryos, we provide evidence that GAF binding establishes competence for rapid activation upon mitotic exit.

Dates et versions

hal-03442288 , version 1 (23-11-2021)

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Citer

Mounia Lagha, Maëlle Bellec, Jérémy Dufourt, George Hunt, Hélène Lenden-Hasse, et al.. The control of transcriptional memory by stable mitotic bookmarking. 2021. ⟨hal-03442288⟩
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