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Chapitre D'ouvrage Année : 2021

Bimolecular Fluorescence Complementation (BiFC) and Multiplexed Imaging of Protein–Protein Interactions in Human Living Cells

Yunlong Jia
Françoise Bleicher
  • Fonction : Auteur
Samir Merabet
Jonathan Reboulet

Résumé

Abstract Flying insects have invaded all the aerial space on Earth and this astonishing radiation could not have been possible without a remarkable morphological diversification of their flight appendages. Here, we show that characteristic spatial expression profiles and levels of the Hox genes Antennapedia ( Antp ) and Ultrabithorax ( Ubx ) underlie the formation of two different flight organs in the fruit fly Drosophila melanogaster . We further demonstrate that flight appendage morphology is dependent on specific Hox doses. Interestingly, we find that wing morphology from evolutionary distant four-winged insect species is also associated with a differential expression of Antp and Ubx . We propose that variation in the spatial expression profile and dosage of Hox proteins is a major determinant of flight appendage diversification in Drosophila and possibly in other insect species during evolution.
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Dates et versions

hal-03437636 , version 1 (20-11-2021)

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Yunlong Jia, Françoise Bleicher, Samir Merabet, Jonathan Reboulet. Bimolecular Fluorescence Complementation (BiFC) and Multiplexed Imaging of Protein–Protein Interactions in Human Living Cells. Multiplexed Imaging, 2350 (1), Springer US; Springer US, pp.173-190, 2021, Methods in Molecular Biology, ⟨10.1007/978-1-0716-1593-5_12⟩. ⟨hal-03437636⟩
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