Kidney inflammaging is promoted by CCR2+ macrophages and tissue-derived micro-environmental factors - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue Cellular and Molecular Life Sciences Année : 2021

Kidney inflammaging is promoted by CCR2+ macrophages and tissue-derived micro-environmental factors

Résumé

Abstract The incidence of disorders associated with low inflammatory state, such as chronic kidney disease, increases in the elderly. The accumulation of senescent cells during aging and the senescence-associated secretory phenotype, which leads to inflammaging, is known to be deleterious and account for progressive organ dysfunction. To date, the cellular actors implicated in chronic inflammation in the kidney during aging are still not well characterized. Using the DECyt method, based on hierarchical clustering of flow cytometry data, we showed that aging was associated with significant changes in stromal cell diversity in the kidney. In particular, we identified two cell populations up-regulated with aging, the mesenchymal stromal cell subset (kMSC) expressing CD73 and the monocyte-derived Ly6C + CCR2 + macrophage subset expressing pro-inflammatory cytokines. Aged CD73 + kMSCs depicted senescence associated features with low proliferation rate, increased DNA damage foci and Ccl2 expression. Using co-cultures experiments, we showed that aged CD73 + kMSC promoted monocyte activation and secretion of inflammatory cytokines albeit less efficiently than young CD73 + kMSCs. In the context of ageing, increased frequency of CD73 + kMSC subpopulations could provide additional niche factors to newly recruited monocytes favoring a positive regulatory loop in response to local inflammation. Interfering with such partnership during aging could be a valuable approach to regulate kidney inflammaging and to limit the risk of developing chronic kidney disease in the elderly.
Fichier principal
Vignette du fichier
Lefèvre.pdf (3.76 Mo) Télécharger le fichier
Origine Fichiers éditeurs autorisés sur une archive ouverte

Dates et versions

hal-03435576 , version 1 (19-11-2021)

Identifiants

Citer

Lise Lefèvre, Jason Iacovoni, Hélène Martini, Julie Bellière, Damien Maggiorani, et al.. Kidney inflammaging is promoted by CCR2+ macrophages and tissue-derived micro-environmental factors. Cellular and Molecular Life Sciences, 2021, 78 (7), pp.3485-3501. ⟨10.1007/s00018-020-03719-0⟩. ⟨hal-03435576⟩
31 Consultations
39 Téléchargements

Altmetric

Partager

Gmail Mastodon Facebook X LinkedIn More