The interactions between gaz/liquid interfaces (bubbles) and cells are involved in many bioprocesses. For example in bioreactors, breathing microorganisms interact with their growth medium but also with the gases present in the medium under the form of bub- bles. While many studies are dedicated to the modelling of such processes, none of them have yet looked into the interactions between the cells and the bubbles. Thus questioning these interactions is highly original, and provides relevant data that can be used in many biotechnological applications. But accessing such interactions presents several technological challenges, the main one being to produce microsized bubbles, stable over time. In this pre- sentation, we show recent developments in which we produce stable bubbles using FluidFM technology that combines AFM with microfluidic AFM probes1. In this system, a micro- sized microfluidic channel is integrated in an AFM cantilever and connected to a pressure controller system, thus creating a continuous and closed fluidic conduit that can be filled with air, while the tool can be immersed in a liquid environment. An aperture at the end of the cantilever allows the air to be pushed out of the probe into the liquid, resulting in the creation of a bubble. Force feedback is then ensured by a standard AFM laser detection system that measures the deflection of the cantilever and thus, interactions can be probed directly with cells. Finally, the bubbles produced using this technique can be functionalized with surfactants, which allows to modulate the interactions between the bubble and cells.