Sumo-mediated recruitment allows timely function of the Yen1 nuclease in mitotic cells - Archive ouverte HAL Accéder directement au contenu
Pré-Publication, Document De Travail Année : 2021

Sumo-mediated recruitment allows timely function of the Yen1 nuclease in mitotic cells

Hugo Dorison
Ibtissam Talhaoui
Gerard Mazón

Résumé

Abstract The modification of DNA damage response proteins with Sumo is an important mechanism to orchestrate a timely and orderly recruitment of repair factors to damaged sites. After replication stress and double-strand break formation a number of repair factors are Sumoylated and interact with other Sumoylated factors, including the nuclease Yen1. Yen1 plays a critical role to ensure genome stability and unperturbed chromosome segregation by removing covalently linked DNA intermediates that are formed by homologous recombination. Here we show how this important role of Yen1 is dependent on interactions mediated by non-covalent binding to Sumoylated partners. Mutations in the motifs that allow Sumo-mediated recruitment of Yen1 impair its ability to resolve DNA intermediates and result in increased genome instability and chromosome mis-segregation.
Fichier principal
Vignette du fichier
manuscript.pdf (3.5 Mo) Télécharger le fichier
Origine : Fichiers produits par l'(les) auteur(s)

Dates et versions

hal-03419397 , version 1 (17-11-2021)

Identifiants

Citer

Hugo Dorison, Ibtissam Talhaoui, Gerard Mazón. Sumo-mediated recruitment allows timely function of the Yen1 nuclease in mitotic cells. 2021. ⟨hal-03419397⟩
16 Consultations
21 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More