Trapping and structural characterisation of a covalent intermediate in vitamin B 6 biosynthesis catalysed by the Pdx1 PLP synthase
Résumé
The Pdx1 enzyme catalyses condensation of two carbohydrates and ammonia to form pyridoxal 5-phosphate (PLP) via an imine relay mechanism of carbonyl intermediates. The I333 intermediate characterised here using structural, UV-vis absorption spectroscopy and mass spectrometry analyses rationalises stereoselective deprotonation and subsequent substrate assisted phosphate elimination, central to PLP biosynthesis.
Domaines
Biologie structurale [q-bio.BM]
Fichier principal
Rodrigues_RSCChemBiol_2021_Pdx1.3_I333_formatted-online.pdf (2.29 Mo)
Télécharger le fichier
Origine : Fichiers éditeurs autorisés sur une archive ouverte