Enormous progress has been made in pulsed electric field-based therapies since J. Teissié reported the occurrence of electric field-induced transient pores in phospholipid bilayer vesicles in 1981. The transient pores, which occurred upon application of short (microsecond) external electric field pulses were attributed to the dielectric breakdown of the bilayer structure and did not damage the lipid membrane. While the term “electroporation” (also known as electropermeabilization or the formation of transient permeant structures) took some time to anchor within the vocabulary of the entire scientific community, experiments showed that pulsed electric field could have numerous biological consequences. Among them, the induction of ATP synthesis, onset of cell fusion, generation of cell hybrids, induction of cytoskeletal reorganization, transfection of cells, delivery of molecules including cytotoxic drugs to cells, and anticancer therapy set the bases for therapeutic applications.