A wide range of materials including mineral dust, soil dust, and bioaerosols have been shown to act as ice nuclei in the atmosphere. During atmospheric transport, these materials can become coated with inorganic and organic solutes which may impact their ability to nucleate ice. While a number of studies have investigated the impact of solutes at low concentrations on ice nucleation by mineral dusts, very few studies have examined their impact on non-mineral dust ice nuclei. We studied the effect of dilute (NH4)2SO4 solutions (0.05 M) on immersion freezing of a variety of non-mineral dust ice-nucleating substances (INSs) including bacteria, fungi, sea ice diatom exudates, sea surface microlayer substances, and humic substances using the droplet-freezing technique. We also studied the effect of (NH4)2SO4 solutions (0.05 M) on the immersion freezing of several types of mineral dust particles for comparison purposes. (NH4)2SO4 had no effect on the median freezing temperature (ΔT50) of 9 of the 10 non-mineral dust materials tested. There was a small but statistically significant decrease in ΔT50 (−0.43 ± 0.19 ∘C) for the bacteria Xanthomonas campestris in the presence of (NH4)2SO4 compared to pure water. Conversely, (NH4)2SO4 increased the median freezing temperature of four different mineral dusts (potassium-rich feldspar, Arizona Test Dust, kaolinite, montmorillonite) by 3 to 9 ∘C and increased the ice nucleation active site density per gram of material (nm(T)) by a factor of ∼ 10 to ∼ 30. This significant difference in the response of mineral dust and non-mineral dust ice-nucleating substances when exposed to (NH4)2SO4 suggests that they nucleate ice and/or interact with (NH4)2SO4 via different mechanisms. This difference suggests that the relative importance of mineral dust to non-mineral dust particles for ice nucleation in mixed-phase clouds could potentially increase as these particles become coated with (NH4)2SO4 in the atmosphere. This difference also suggests that the addition of (NH4)2SO4 (0.05 M) to atmospheric samples of unknown composition could potentially be used as an indicator or assay for the presence of mineral dust ice nuclei, although additional studies are still needed as a function of INS concentration to confirm the same trends are observed for different INS concentrations than those used here. A comparison with results in the literature does suggest that our results may be applicable to a range of mineral dust and non-mineral dust INS concentrations.