Impaired development of hippocampal mossy fibre synapses in mouse mutants for the presynaptic scaffold protein Bassoon - Archive ouverte HAL Accéder directement au contenu
Article Dans Une Revue The Journal of Physiology Année : 2010

Impaired development of hippocampal mossy fibre synapses in mouse mutants for the presynaptic scaffold protein Bassoon

Frederic Lanore
Christophe Blanchet
  • Fonction : Auteur
Anna Fejtova
  • Fonction : Auteur
Paulo Pinheiro
  • Fonction : Auteur
Karin Richter
  • Fonction : Auteur
Detlef Balschun
  • Fonction : Auteur
Eckart Gundelfinger
  • Fonction : Auteur
Christophe Mulle
  • Fonction : Auteur
  • PersonId : 913077

Résumé

Bassoon, a protein highly concentrated at the synaptic active zone, is thought to participate in the organization of the cytomatrix at the site of neurotransmitter release. Bassoon is amongst the first proteins to accumulate at newly formed synaptic junctions, raising the question of the functional role of this protein in the early stages of synaptic development. Here we show that the course of synaptic maturation of hippocampal mossy fibre (MF) synapses (glutamatergic synapses with multiple release sites) is markedly altered during the first 2 weeks of postnatal development in mutant mice lacking the central region of Bassoon (Bsn −/− mice). At postnatal day 7 (P7), Bsn −/− mice display large amplitude MF-EPSCs with decreased paired pulse ratios, an abnormality which may be linked to deficits in the organization of the presynaptic active zone. Surprisingly, 1 week later, decreased MF-EPSCs amplitude is observed in Bsn −/− mice, consistent with the inactivation of a subset of synaptic release sites. Finally, at more mature states a decreased posttetanic potentiation is observed at MF-synapses. These results support the notion that Bassoon is important for organizing the presynaptic active zone during the postnatal maturation of glutamatergic synapses.

Dates et versions

hal-03367925 , version 1 (10-11-2021)

Identifiants

Citer

Frederic Lanore, Christophe Blanchet, Anna Fejtova, Paulo Pinheiro, Karin Richter, et al.. Impaired development of hippocampal mossy fibre synapses in mouse mutants for the presynaptic scaffold protein Bassoon. The Journal of Physiology, 2010, 588, pp.2133 - 2145. ⟨10.1113/jphysiol.2009.184929⟩. ⟨hal-03367925⟩

Collections

CNRS
4 Consultations
0 Téléchargements

Altmetric

Partager

Gmail Facebook X LinkedIn More